Agenda

Monday, December 11, 2017
1:00 PM - 2:00 PM Antibody Production: Polyclonals, Monoclonals, and New Developments
Presented by Debra Freeman, MilliporeSigma

Antibodies and immunohistochemistry are each equally fascinating. How do we move from an amino acid sequence to a functioning antibody in the lab? We will discuss a history of the evolution of antibody development, manufacturing and production techniques. Looking at the pros and cons of polyclonal and monoclonal antibodies and how each are produced. This workshop will dive into the move from the simplest form of antibody production to some of the latest technology in monoclonal antibody production.
SOLD OUT
Tuesday, December 12, 2017
1:00 PM - 2:00 PM Simple Tools for 3-Dimensional Image Processing
Presented by Sean McKinney, Ph.D., Stowers Institute for Medical Research

Large, 3-dimensional datasets are commonplace now with platforms like selective plane illumination (SPIM), spinning disk microscopy, and serial section imaging. Reconstructing 3-dimensional visualizations from these datasets can be cpu and memory intensive, but can be done with relatively simple tools that are freely available. Here we will look at reconstructing a sectioned dataset using FiJi. We will also take a look at finding and quantifying cells automatically on a 3-dimensional confocal dataset.
SOLD OUT
Wednesday, December 13, 2017
1:00 PM - 2:00 PM Multiplexing on Animal and Xenograft Tissue, All Things Considered 
Presented by Andrea Transou, HT(ASCP), Henry Ford Health System

Every doctor and investigator desires to know three things: can the task required be completed, how long will it take, and how much will it cost? Multiplexing is an immunohistochemical technique that allows for the visualization of multiple antigens on the same slide. The ability to visualize multiple antigens through fluorescence or chromogenic staining saves time, money, tissue, and reagents. In order to do this successfully, it is necessary to know how to properly develop a protocol. Issues such as background staining, non-specific staining, species to species reactivity, and a lack of protocol development tactics can hinder the efficient use of multiplexing. This webinar will walk histology professionals through antibody and reagent selection for all tissue types with consideration of species to species reactivity. It will also address concerns of proper control material selection, antigen retrieval, chromogen usage, and stain order. Developing a successful multiplexing protocol can be a daunting task, but with the tools from this webinar anyone can accomplish it.
SOLD OUT
Thursday, December 14, 2017
1:00 PM - 2:00 PM Superresolution Expansion Microscopy
Presented by Yongfu Wang, Ph.D., Stowers Institute for Medical Research

Recently, expansion microscopy (ExM) has become a very popular and powerful tool for examining protein and RNA in cultures, biological tissue, and clinical specimens, allowing more detailed views of molecular structures using light microscopy. At the Stowers Institute for Medical Research, we have developed a protocol combining ExM with structured illumination microscopy (super resolution ExM, sExM). The focus of this webinar will be to discuss the principles and science of ExM, the strategy of sExM, applications, and troubleshooting.
Friday, December 15, 2017
1:00 PM - 2:00 PM Developing Immunohistochemical (IHC) Assays for Animal Models of Disease
Presented by Elizabeth A. Chlipala, BS, HTL(ASCP)QIHC, Premier Laboratory, LLC

In a wide variety of research settings we are tasked to develop immunohistochemical (IHC) assays that will be specifically used for animal models. This webinar will discuss the detailed methods necessary to successfully develop and verify these assays for both well characterized and novel targets. Topics discussed will include;

1. The importance of creating a collaborative environment between the researcher/pathologist and the histology laboratory.

2. The value of understanding the animal model and the type of data that is to be generated from the histology process.

3. How to research and select the right antibody and control tissues.

4. The use of pilot samples for protocol optimization.

5. How to develop standardized study specific methods for proper tissue collection, fixation, processing, sectioning and IHC staining.
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